A5 - Enzyme Immobilization

(A. Rosevear, J. F. Kennedy and J. M. S. Cabral22)

Enzyme immobilization is a process where the movement of the enzyme is severely restricted in space in such a way that its catalytic activity is still preserved. Enzyme immobilization allows continuous use and reuse of the catalyst.

Some advantages of the immobilized enzymes over their soluble forms are:

Despite this advantages industrial application is still limited by:

The choice of the matrix is very important for the good performance of an immobilized enzyme system. It is then desirable that an enzyme carrier possesses:

Alternatively it is also possible to immobilize, either viable or non-viable, whole cells. This is of great help when cofactors and/or several enzymes are needed at the same time. However, side reactions are possible and thus less pure products are obtained.

Classification of Immobilized Enzymes

The term immobilized enzyme includes:

The classification of the immobilization methods for enzymes presented here is based on the nature of the interaction and the nature of the support used.

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