Margarida Maria Portela Correia dos Santos Romão
Associate Professor
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Research Unit
Education
Habilitation (D.Sc.), Chemistry, Instituto Superior Técnico (2004)
Ph.D., Chemistry, Instituto Superior Técnico (supervision: M.L.Simões Gonçalves, 1987)
Diploma, Chemical Engineering (major in Chemistry and Processes), Instituto Superior Técnico (1978)
Academic Positions
Associate Professor, DEQB, Instituto Superior Técnico since 2002
Assistant Professor, DEQ, Instituto Superior Técnico, 1987-2002
Teaching Assistent, DEQ, Instituto Superior Técnico, 1979-1987
Teaching Assistent, FCT, Universidade Nova de Lisboa, 1978-1979
Demonstrator, DEQ, Instituto Superior Técnico, 1977-1978
Teaching assignments
At Instituto Superior Técnico she has lectured several subjects from the scientific areas of Analytical Chemistry, Instrumental Methods of Analysis, Electrochemistry and Structural Biology in the graduation courses of Chemistry, Chemical Engineering and Biological Engineering.
She has also lectured the same subjects in post graduation courses at IST and other Faculties.
Others assignments at IST
Director of Laboratory of Analysis of IST (LAIST)
Domain of Specialization:
Electrochemistry Applied to Physical-Chemical Processes in Natural Systems
Research Activities:
Environmental Chemistry: of metal ions in natural waters, soils and sediments with special emphasis on their speciation (redox and dynamic speciation) to evaluated their mobility, toxicity and availability. Speciation of metallic nanoparticles in the environment
Bioelectrochemistry: Electrodes for redox proteins. Direct and mediated electrochemistry of redox proteins and enzymes. Kinetics and energetics of e-transfer and coupled interactions with redox partners and substrates in metalloproteins. Study of intra and intermolecular electron transfer (recognition). Study of of substrate/enzyme interactions (catalysis).
Development and application of analytical methodologies with special emphasis on electroanalysis.
Others Scientific Activities
Member of the technical committee TC 24 “Chemical Measurements” of the International Measurement Confederation - IMEKO.
Recent Funded Projects
PTDC/AAC-AMB/111998/2009 - "Metallic nanoparticles in the environment: dynamic speciation and toxicology" (in course)
PTDC/BIAPRO/109796/2009 - "Insights into novel bacterial cytochrome c peroxidases from pathogenic bacteria, Neisseria gonorrhoeae and Escherichia coli", IST Participant Institution (in course)
POCI/QUI/55743/2004. - “Mechanisms and Energetics of the electron transport in metlloproteins by dynamic voltammetry” (PI) (2005-2009)
POCTI/QUI/42277/2002. - “Dynamic Electrochemical studies in electron transfer proteins” (2002-2005)
POCTI/1999/MGS/35653. - “Modelation of Metal-Ligand interactions in soils and plants” (2001-2004)
Publications:
2006-present
"Dynamic Modelling
of Nickel Complexation in Xylem sap of Quercus ilex: A Voltammetric Study"
M.M.Correia dos Santos, S.Alves, M.L.Simões Gonçalves, C.Nabais, Electroanalysis,
18 (2006) 814. doi: 10.1002/elan.200503446
"Redox Chemistry
of low-pH formas of tetrahemic cytochrome c3"
M.Santos, M.M.Correia dos Santos, M.L.Simões Gonçalves, C.Costa, J.C.Romão,
J.J.G.Moura, J.Inorg.Biochem., 100 (2006) 2009-2016.
doi:10.1016/j.jinorgbio.2006.09.013
“Lead sorption
to selected Portuguese soils”
M.A.Trancoso, M.M.Correia dos Santos, M.L.Simões Gonçalves, European Journal of
Soil Science, 58 (2007) 854-863.doi:10.1111/j.1365-2389.2006.00876.x
“Mediated
catalysis of Paracocus pantotrophus cytochrome c peroxidise by
Paracocus pantotrophus pseudoazurin – kinetics of intermolecular electron
transfer”
P.M.Paes de Sousa, S.R.Pauleta, M.L.Simões Gonçalves, G.W. Pettigrew, I.Moura,
M.M.Correia dos Santos, J.J.G.Moura, J.Biol.Inorg.Chem 12 (2007) 691-698.
doi:10.1007/s00775-007-0219-9
“Kinetics and Mechanism of
Ni(II) Chelation in Model and Real Solutions of Xylem Sap of Quercus ilex".
M. M.Correia dos
Santos, S. Alves, M.L.imões Gonçalves.
Electroanalysis
19 (2007) 2351 – 2361. doi:10.1002/elan.200703972
"Adaptation of
a commercial ion selective fluoride electrode to a tubular configuration for
analysis by flow methodologies"
António C.L. Conceição, M.M. Correia dos Santos, M.L.S. Simões Gonçalves,
Talanta 76 (2008) 107–110. doi:10.1016/j.talanta.2008.02.010.
"Benefits of
membrane electrodes in the electrochemistry of metalloproteins: mediated
catalysis of Paracoccus pantotrophus cytochrome c peroxidase by
horse cytochrome c: a case study"
P. M. Paes de Sousa, S. R. Pauleta, D. Rodrigues, M. L. Simões Gonçalves,G. W.
Pettigrew, I. Moura, J. J. G. Moura ,M. M. Correia dos Santos, J Biol Inorg Chem
13 (2008) 779–787. doi:10.1007/s00775-008-0365-8
"Enzymatic
biotransformation of the azo dye Sudan Orange G with bacterial CotA-laccase"
Luciana Pereira, Ana V. Coelho, Cristina A. Viegas, M. M. Correia dos Santos,
Maria Paula Robaloc, Lígia O. Martins, Journal of Biotechnology 139 (2009) 68–77
doi:10.1016/j.jbiotec.2008.09.001
"Evaluation of
measurement uncertainties for the determination of total metal content in soils
by atomic absorption spectrometry"
Sheila Alves, M.M. Correia dos Santos, M.A.Trancoso, Accreditation and Quality
Assurance, 14 (2009) 87-93. doi:10.1007/s00769-008-0484-5
"Determination of Nickel, Calcium and Magnesium in xylem saps by FAAS using a
microsampling technique”
Sheila Mónica de Jesus Raposo Alves, M.L.Simões Gonçalves, M.M. Correia dos
Santos, Phytochemical Analysis - 20 (2009) 365-369. doi:10.1002/pca.1135
"Electrochemical oxidation of the synthetic anthocyanin analogue 4-methyl-7,8-
dihydroxyflavylium salt"
Margarida Cabral Moncada, Maria Fernanda de Mesquita, M.M.Correia dos Santos, J.
Electroanal. Chem., 636 (2009) 60-67. doi:10.1016/j.jelechem.2009.09.004
"Artefacts induced on c-type heam proteins by electrodes surfaces"
P.M.Paes de Sousa, S.R.Pauleta, D.Rodrigues, M.L.Simões Gonçalves, G.W.Petrigrew,
I.Moura, J.J.G.Moura, M.M.Correia dos Santos, J Biol Inorg Chem 16 (2011) 209. doi:
10.1007/s00775-010-0717-z
Analysis of the activation mechanism of Pseudomonas stutzeri cytochrome
c peroxidase through an electron transfer chain
P. M. Paes de Sousa, D. Rodrigues, C. G. Timóteo,
M. L. Simões Gonçalves, G. W. Pettigrew, I. Moura,
J. J. G. Moura , M. M. Correia dos Santos, J Biol Inorg
Chem (2011), in publication
"Nickel Speciation in the
Xylem Sap of the Hyperaccumulator Alyssum serpyllifolium ssp. lusitanicum
Growing on Serpentine Soils of Northeast Portugal"
Sheila Alves, C. Nabais, M. L. Simões Gonçalves, M. M. Correia dos Santos,
Journal Plant Physiology, (2010), submited.
"A nickel availability study in serpentinized areas of Portugal"
Sheila Alves, M.A.Trancoso, M.L.Simões Gonçalves, M.M. Correia dos Santos,
Geoderma, (2010), submited.
“Inorganic
iodine speciation in seawater of two western Portuguese beaches – a preliminary
study”
M.Pimpão,
R.Bernardino I,Pinto, S.Mendes; M.G.Almeida, M.M.Correia dos Santos, Intern.
J.Environ.Anal.Chem., (2010),
submited.
2000-2005
“A
voltammetric study of the complexation of copper by the psychoactive compounds
1,4-Benzodiazepines”
M.M.Correia dos Santos, V.Famila; M.L.Simões Gonçalves, Electroanalyis, 12
(2000) 216.
doi:10.1002/(SICI)1521-4109(200002)12:3
“Gradient Flow
Titration for the determination of fluoride ion in natural waters”
A.C. Lopes Conceição, M.M.Correia dos Santos, M.L.Simões Gonçalves,
FernandoJ.V.Santos, Talanta, 50 (2000) 1245.
10.1016/S0039-9140(99)00236-2
“Electrochemical
studies of rubredoxin from Desulfovibrio.vulgaris at modified electrodes”
M.M. Correia dos Santos, P.M.Paes de Sousa, M.L.Simões Gonçalves, C.Ascenso,
I.Moura, J.J.G.Moura, J. Electroanal.
Chem., 501 (2001) 173.
doi:10.1016/S0022-0728(00)00521-0
“Speciation of inorganic
arsenic in natural waters by Square Wave Cathodic Stripping Voltammetry”
C.M.Barra, M.M.Correia dos Santos, Electroanalyis, 13 (2001) 1098.
doi:10.1002/1521-4109(200109)13:3
”Interaction of
lead (II) with sediment particles– a mercury microelectrode study”
M.M.Correia dos Santos, M.F.Vilhena, M.L.Simões Gonçalves, Anal. Chim. Acta, 441
(2001)191. doi:10.1016/S0003-2670(01)01108-4
“Copper
psychoactive drugs complexes: a voltammetric approach to the complexation by 1,
4 benzodiazepines”
M.M.Correia dos Santos, Vila Famila, M.L.Simões Gonçalves, Analytical
Biochemistry, 303 (2002) 111 doi: 10.1006/abio.2002.5580
“Square wave
voltammetric techniques for determination of the psychoactive
1,4-benzodiazepines drugs”
M.M.Correia dos Santos, V.Famila, M.L.Simões Gonçalves, Anal.Bioanal.Chem., 372
(2002) 1047. doi: 10.1007/s00216-002-1535-0
“Electrochemical
studies on small electron transfer proteins using membrane electrodes”
M.M. Correia dos Santos, P.M.Paes de Sousa, M.L.Simões Gonçalves, L. Krippahl,
J.J.G.Moura, É.Lojou, P.Bianco, J. Electroanal. Chem., 541 (2003) 153.
doi:10.1016/S0022-0728(02)01427-4
“Quality assurance
program for the chemical characterization of soils”
M. Trancoso, M.M. Correia dos Santos, M.L. Simões Gonçalves,
Accreditation and Quality Assurance, 8 (2003) 323. doi:
10.1007/s00769-003-0608-x
“Flow amperometric
determination of pharmaceuticals with on-line electrode surface renewal”
R.I.L. Catarino, A.L. Conceição, V. Freitas, M.B. Quinaz Garcia, M.L. Simões
Gonçalves, J.L.C. Lima, M.M. Correia dos Santos, Journal of Pharmaceutical and
Biomedical Analysis, 33 (2003) 571. doi: 10.1016/S0731-7085(03)00318-2
“Direct
Electrochemistry of the Desulfovibrio gigas aldehyde oxidoreductase”
M.M. Correia dos Santos, P.M.Paes de Sousa, M.L.Simões Gonçalves, M.J.Romão,
J.J.G. Moura, I. Moura, Eur.J.Biochem. 271 (2004) 1329. doi:
10.1111/j.1432-1033.2004.04041.x
“Intraspecific
Variation of mercury contamination in chicks of Black-Winged Stilt (Himantopus
himantopus) in Coastal Wetlands from Southwestern Europe"
P.C. Tavares, L.R. Monteiro,R.J. Lopes, M.M. Correia dos Santos, R.W. Furness,
Bulletin of Environmental Contamination and Toxicology, 72 (2004) 437
doi:10.1007/s00128-004-0265-8
“Lead
adsorption on a soil: a polarografic study”
M.A. Trancoso, M.M.Correia dos Santos, M.L.Simões Gonçalves, Electroanalysis, 16
(2004) 1024-1032. doi: 10.1002/elan.200302891
Before 2000
"Determination of stability
constants of quasi-reversible systems: copper chlorocomplexes in seawater
conditions"
M.L. Simões Gonçalves, M.M. Correia dos Santos, J. Electroanal. Chem., 143
(1983) 397. doi:10.1016/S0022-0728(83)80276-9
"Cadmium complexes of
aminoacids in seawater conditions"
M.L. Simões Gonçalves, M.M. Correia dos Santos, J. Electroanal.
Chem., 163
(1984) 315. doi:10.1016/S0022-0728(84)80059-5
"Electroanalytical
chemistry of cadmium complexes of aminoacids at the ionic strength of seawater
(0.70M NaClO )- Part II"
M.L. Simões Gonçalves, M.M. Correia dos Santos, J. Electroanal. Chem., 187
(1985) 333. doi:10.1016/0368-1874(85)85788-9
"Electroanalytical
chemistry of copper, lead and zinc complexes of amino-acids at the ionic
strength of seawater (0.70M NaClO4 ).
Part III"
M. M. Correia dos Santos, M. L. Simões Gonçalves, J. Electroanal. Chem., 208
(1986) 137. doi:10.1016/0022-0728(86)90302-5
"Simulations of a
Natural Interface"
A. M. Mota, M.L. Simões Gonçalves, M. M. Correia dos Santos, Port.
Elect. Acta, 5
(1987), 191.
"Flow-Injection
Titration of Alkalinity in Natural Waters"
D. R. Turner,
S. Knox, M. Whitfield, M. M. Correia dos Santos, Analytical Proceedings, 24
(1987) 359-364.
doi:10.1039/AP9872400359
"Adsorption of
aminoacids on the mercury electrode and kinetics of dissociation of their Cu(II),
Pb(II), Cd(II) and Zn(II) complexes at the ionic strength of seawater (0.70M
NaClO4)”
M.L. Simões Gonçalves, M.M. Correia dos Santos, The Science of the Total Env.,
76 (1988) 1. doi:10.1016/0048-9697(88)90278-1
"Application of
Cyclic Voltammetry to a CE Mechanism in a Natural Water"
M.L. Simões Gonçalves, M.M. Correia dos Santos, Port. Elect. Acta, 7 (1989) 37.
" An Integrated
Gradient Chamber and Potentiometric Detector for flow injection analysis : Part
1. Assessment of the system"
D.R. Turner, S. Knox, M. Whitfield, M.M. Correia dos Santos, C. Pescada, M.L.
Gonçalves, Anal. Chim.
Acta, 222 (1989) 229. doi:10.1016/S0003-2670(00)83280-8
" An Integrated
Gradient Chamber and Potentiometric Detector for flow injection analysis : Part
2. Measurement of acidity constants of weak acids "
D.R. Turner, S. Knox, M. Whitfield, M.M. Correia dos Santos, C. Pescada, M.L.
Gonçalves, Anal. Chim.
Acta, 222 (1989) 239. doi:10.1016/S0003-2670(00)83281-X
"Reduction of
Copper(II) in the Presence of Monoamines at the Ionic Strength of Seawater"
M.M. Correia dos Santos, M.L. Simões Gonçalves, Analytical Proceedings, 28
(1991) 72-81 Doi:10.1039/AP9912800072
"Voltammetric
Studies of Copper(I) and Copper(II) with Monoamines at the Ionic Strength of the
Seawater"
M.M. Correia dos Santos, M.L. Simões Gonçalves, Electroanalysis, 3 (1991) 131.
doi:10.1002/elan.1140030210
"Rapid pK
Measurements for Multibasic Acids by Gradient FIA Titration",
D.R. Turner, M.M. Correia dos Santos,P.Coutinho, M.L. Simões Gonçalves, S. Knox,
Anal. Chim. Acta, 258 (1992) 259. doi:10.1016/0003-2670(92)85100-K
"Determination of Stability
Constants Using a Mercury Microelectrode and Steady-State Voltammetry "
M.M. Correia dos Santos, M.L. Simões Gonçalves, Electrochim. Acta, 37 (1992)
1413. doi:10.1016/0013-4686(92)87015-R
"Determination of Stability
Constants
Using Normal Pulse Voltammetry at Microelectrodes "
K. Wikial, M.M. Correia dos Santos, J. Osteryoung, Electrochim. Acta, 38 (1993)
1555. doi:10.1016/0013(93)80286-C
"Kinetics of
Dissociation of Copper (II) Proline Complexes by Cyclic Voltammetry with NAFION
- Coated Electrode"
M.M. Correia dos Santos, Sofia Capelo, M.L.S. Simões Gonçalves, J. Electroanal.
Chem., 364 (1994) 171. doi:10.1016/0022-0728(93)02948-H
"Rapid
Determination of Complexing Stability Constants by Gradient Flow Injection
Titration"
A.L. Conceição, M.L. Simões Gonçalves, M.M. Correia dos Santos, Anal. Chim. Acta,
302 (1995) 97. doi:10.1016/0003-2670(94)00434-N
"Cadmium and
Lead Complexation by Anodic Stripping Voltammetry with a Mercury Microelectrode"
M.M. Correia dos Santos, M.L. Simões Gonçalves, S. Capelo, Electroanalysis, 8
(1996) 178 doi:10.1002/elan.1140080215
"Voltammetric Studies of
Purine Bases and Purine Nucleosides with Copper(II)"
M.M. Correia dos Santos, C.M.L. Freire Lopes, M.L. Simões Gonçalves,
Bioelectrochem. Bioenerg., 39 (1996) 55. doi:10.1016/0302-4598(95)01857-3
"Study of CE mechanisms by
square wave voltammetry: Cd(II) nitrilotriacetic acid and Cd(II)- aspartic acid
systems"
M. M. Correia dos Santos, M.L. Simões Gonçalves, J.C. Romão, J. Electroanal.
Chem., 413 (1996) 97. doi:10.1016/0022-0728(96)04614-1
“Flow injection-assisted
optical sensor for determination of iron(II) and iron(III) in natural water”
A.L.Conceição, M.T.Tena, M.M.Correia dos Santos, M.L.Simões Gonçalves, M.D.Luque
de Castro, Anal. Chim. Acta, 343 (1997) 191. doi:10.1016/S0003-2670(96)00595-8
“Electrochemical behavior at
macro and microelectrodes of cytochromes c and c3 from
D.vulgaris - A comparative study”
P.M.Paes de Sousa, M.M.Correia dos Santos, M.L.Simões Gonçalves, Port. Elect.
Acta, 15 (1997) 407.
“Copper complexation by
cytosine and its nucleoside”
M.M.Correia dos Santos, P.M.P.Sousa, A.M.M.Modesto, M.L.Simões Gonçalves,
Bioelectrochem.
Bioenerg, 45
(1998) 267. doi:10.1016/S0302-4598(97)00102-5
"Electrochemical
Studies on c-type Cytochromes at Microelectrodes”
M.M. Correia dos Santos, P.M.P. Sousa, M.L. Simões Gonçalves, H. Lopes, I.
Moura, J. Moura, J. Electroanal. Chem., 464 (1999) 76.
doi:10.1016/S0022-0728(98)00474-4
“Thermodynamics
of uptake of Cadmium by Chlorella Marina”
M.C.Ezequil Rollemberg, M.L.Simões Gonçalves, M.M.Correia dos Santos,
Bioelectrochem. Bioenerg., 48 (1999) 61. doi:10.1016/S0302-4598(98)00220-
“Speciation of
copper in the presence of bromazepam”,
V.Famila, M.M.Correia dos Santos, M.L.Simões Gonçalves, Port.
Elect.
Acta, 17 (1999)
165.
