Margarida Maria Portela Correia dos Santos Romão

Professora Associada com Agregação 


Departamento de Engenharia Química e Biológica
Instituto Superior Técnico (IST)
Universidade Técnica de Lisboa
Av. Rovisco Pais
P - 1049-001 Lisboa Codex
Portugal
telefone: (+351) 21 841 92 72
fax: (+351) 21 846 44 55 / 7
email: mcsantos @ ist.utl.pt


 

Unidade de Investigação 

         Centro de Química Estrutural


 

Formação Académica


 

Carreira Académica   

 


Ensino

No Instituto Superior Técnico tem leccionado vários assuntos nas áreas científicas de Química Analítica, Métodos Instrumentias de Análise, Electroquímica e Biologia Estructural nas licenciaturas de Química, Engenharia Química e Engenharia Biológica.

Tem também leccionado os mesmos tópicos em cursos de pós-graduação no Instituto Superior Técnico e por convite em outras Faculdades e Centros de Investigação

Outras actividades no IST


 

Interesses Científicos

Domínio de especialização:  

Electroquímica aplicada ao estudo de processos físico-químicos nos sistemas naturais

actividades de investigação

 Outras actividades científicas

 Membro do Comité Técnico TC24 "Medidas Químicas"  da organização  International Measurement Confederation  - IMEKO.

Annual Research Report 2007


Projectos Financiados Recentes

PTDC/AAC-AMB/111998/2009 - "Metallic nanoparticles in the environment: dynamic speciation and toxicology", em curso.

PTDC/BIAPRO/109796/2009  - "Insights into novel bacterial cytochrome c peroxidases from pathogenic bacteria, Neisseria gonorrhoeae and Escherichia coli", IST  Instituição Participante, em curso.

POCI/QUI/55743/2004. - “Mechanisms and Energetics of the electron transport in metlloproteins by dynamic voltammetry” (PI) (2005-2009)

POCTI/QUI/42277/2002. - “Dynamic Electrochemical studies in electron transfer proteins” (2002-2005)

POCTI/1999/MGS/35653.  - “Modelation of Metal-Ligand interactions in soils and plants” (2001-2004)


Publicações

2006 - PRESENTE

"Dynamic Modelling of Nickel Complexation in Xylem sap of Quercus ilex: A Voltammetric Study"
M.M.Correia dos Santos, S.Alves, M.L.Simões Gonçalves, C.Nabais, Electroanalysis, 18 (2006) 814. doi: 10.1002/elan.200503446

 "Redox Chemistry of low-pH formas of tetrahemic cytochrome c3"
M.Santos, M.M.Correia dos Santos, M.L.Simões Gonçalves, C.Costa, J.C.Romão, J.J.G.Moura, J.Inorg.Biochem., 100 (2006) 2009-2016. doi:10.1016/j.jinorgbio.2006.09.013 

“Lead sorption to selected Portuguese soils”
M.A.Trancoso, M.M.Correia dos Santos, M.L.Simões Gonçalves, European Journal of Soil Science, 58 (2007) 854-863.doi:10.1111/j.1365-2389.2006.00876.x

“Mediated catalysis of Paracocus pantotrophus cytochrome c peroxidise by Paracocus pantotrophus pseudoazurin – kinetics of intermolecular electron transfer”
P.M.Paes de Sousa, S.R.Pauleta, M.L.Simões Gonçalves, G.W. Pettigrew, I.Moura, M.M.Correia dos Santos, J.J.G.Moura, J.Biol.Inorg.Chem 12 (2007) 691-698. doi:10.1007/s00775-007-0219-9

“Kinetics and Mechanism of Ni(II) Chelation in Model and Real Solutions of Xylem Sap of Quercus ilex".
M.
M.Correia dos Santos, S. Alves, M.L.imões Gonçalves. Electroanalysis 19 (2007) 2351 – 2361. doi:10.1002/elan.200703972

"Adaptation of a commercial ion selective fluoride electrode to a tubular configuration for analysis by flow methodologies"
António C.L. Conceição, M.M. Correia dos Santos, M.L.S. Simões Gonçalves, Talanta 76 (2008) 107–110. doi:10.1016/j.talanta.2008.02.010.

"Benefits of membrane electrodes in the electrochemistry of metalloproteins: mediated catalysis of Paracoccus pantotrophus cytochrome c peroxidase by horse cytochrome c: a case study"
P. M. Paes de Sousa, S. R. Pauleta, D. Rodrigues, M. L. Simões Gonçalves,G. W. Pettigrew, I. Moura, J. J. G. Moura ,M. M. Correia dos Santos, J Biol Inorg Chem 13 (2008) 779–787. doi:10.1007/s00775-008-0365-8

"Enzymatic biotransformation of the azo dye Sudan Orange G with bacterial CotA-laccase"
Luciana Pereira, Ana V. Coelho, Cristina A. Viegas, M. M. Correia dos Santos, Maria Paula Robaloc, Lígia O. Martins, Journal of Biotechnology 139 (2009) 68–77 doi:10.1016/j.jbiotec.2008.09.001

"Evaluation of measurement uncertainties for the determination of total metal content in soils by atomic absorption spectrometry"
Sheila Alves, M.M. Correia dos Santos, M.A.Trancoso, Accreditation and Quality Assurance, 14 (2009) 87-93. doi:10.1007/s00769-008-0484-5

"Determination of Nickel, Calcium and Magnesium in xylem saps by FAAS using a microsampling technique”
Sheila Mónica de Jesus Raposo Alves, M.L.Simões Gonçalves, M.M. Correia dos Santos, Phytochemical Analysis - 20 (2009) 365-369. doi:10.1002/pca.1135

"Electrochemical oxidation of the synthetic anthocyanin analogue 4-methyl-7,8- dihydroxyflavylium salt"
Margarida Cabral Moncada, Maria Fernanda de Mesquita, M.M.Correia dos Santos, J. Electroanal. Chem., 636  (2009) 60-67. doi:10.1016/j.jelechem.2009.09.004

"Artefacts induced on c-type heam proteins by electrodes surfaces"
P.M.Paes de Sousa, S.R.Pauleta, D.Rodrigues, M.L.Simões Gonçalves, G.W.Petrigrew, I.Moura, J.J.G.Moura, M.M.Correia dos Santos, J Biol Inorg Chem 16 (2011) 209.  doi: 10.1007/s00775-010-0717-z

Analysis of the activation mechanism of Pseudomonas stutzeri cytochrome c peroxidase through an electron transfer chain
P. M. Paes de Sousa, D. Rodrigues, C. G. Timóteo, M. L. Simões Gonçalves, G. W. Pettigrew, I. Moura, J. J. G. Moura , M. M. Correia dos Santos, J Biol Inorg Chem (2011), in publication

 "Nickel Speciation in the Xylem Sap of the Hyperaccumulator Alyssum serpyllifolium ssp. lusitanicum Growing on Serpentine Soils of Northeast Portugal"
Sheila Alves, C. Nabais, M. L. Simões Gonçalves, M. M. Correia dos Santos, Journal Plant Physiology, (2010), submited.

"A nickel availability study in serpentinized areas of Portugal"
Sheila Alves, M.A.Trancoso, M.L.Simões Gonçalves, M.M. Correia dos Santos, Geoderma, (2010), submited.

“Inorganic iodine speciation in seawater of two western Portuguese beaches – a preliminary study”
M.Pimpão, R.Bernardino I,Pinto, S.Mendes; M.G.Almeida, M.M.Correia dos Santos, Intern.
J.Environ.Anal.Chem., (2010), submited.

2000-2005

 “A voltammetric study of the complexation of copper by the psychoactive compounds 1,4-Benzodiazepines”
M.M.Correia dos Santos, V.Famila; M.L.Simões Gonçalves, Electroanalyis, 12 (2000) 216.
doi:10.1002/(SICI)1521-4109(200002)12:3

“Gradient Flow Titration for the determination of fluoride ion in natural waters”
A.C. Lopes Conceição, M.M.Correia dos Santos, M.L.Simões Gonçalves, FernandoJ.V.Santos, Talanta, 50 (2000) 1245.
10.1016/S0039-9140(99)00236-2

“Electrochemical studies of rubredoxin from Desulfovibrio.vulgaris at modified electrodes”
M.M. Correia dos Santos, P.M.Paes de Sousa, M.L.Simões Gonçalves, C.Ascenso, I.Moura, J.J.G.Moura, J. Electroanal.
Chem., 501 (2001) 173. doi:10.1016/S0022-0728(00)00521-0

“Speciation of inorganic arsenic in natural waters by Square Wave Cathodic Stripping Voltammetry”
C.M.Barra, M.M.Correia dos Santos, Electroanalyis, 13 (2001) 1098.
doi:10.1002/1521-4109(200109)13:3

”Interaction of lead (II) with sediment particles– a mercury microelectrode study”
M.M.Correia dos Santos, M.F.Vilhena, M.L.Simões Gonçalves, Anal. Chim. Acta, 441 (2001)191. doi:10.1016/S0003-2670(01)01108-4

“Copper psychoactive drugs complexes: a voltammetric approach to the complexation by 1, 4 benzodiazepines”
M.M.Correia dos Santos, Vila Famila, M.L.Simões Gonçalves, Analytical Biochemistry, 303 (2002) 111 doi: 10.1006/abio.2002.5580

“Square wave voltammetric techniques for determination of the psychoactive 1,4-benzodiazepines drugs”
M.M.Correia dos Santos, V.Famila, M.L.Simões Gonçalves, Anal.Bioanal.Chem., 372 (2002) 1047. doi: 10.1007/s00216-002-1535-0

“Electrochemical studies on small electron transfer proteins using membrane electrodes”
M.M. Correia dos Santos, P.M.Paes de Sousa, M.L.Simões Gonçalves, L. Krippahl, J.J.G.Moura, É.Lojou, P.Bianco, J. Electroanal. Chem., 541 (2003) 153. doi:10.1016/S0022-0728(02)01427-4

“Quality assurance program for the chemical characterization of soils”
M. Trancoso, M.M. Correia dos Santos, M.L. Simões Gonçal
ves, Accreditation and Quality Assurance, 8 (2003) 323. doi: 10.1007/s00769-003-0608-x

“Flow amperometric determination of pharmaceuticals with on-line electrode surface renewal”
R.I.L. Catarino, A.L. Conceição, V. Freitas, M.B. Quinaz Garcia, M.L. Simões Gonçalves, J.L.C. Lima, M.M. Correia dos Santos, Journal of Pharmaceutical and Biomedical Analysis, 33 (2003) 571. doi: 10.1016/S0731-7085(03)00318-2

“Direct Electrochemistry of the Desulfovibrio gigas aldehyde oxidoreductase”
M.M. Correia dos Santos, P.M.Paes de Sousa, M.L.Simões Gonçalves, M.J.Romão, J.J.G. Moura, I. Moura, Eur.J.Biochem. 271 (2004) 1329. doi: 10.1111/j.1432-1033.2004.04041.x

“Intraspecific Variation of mercury contamination in chicks of Black-Winged Stilt (Himantopus himantopus) in Coastal Wetlands from Southwestern Europe"
P.C. Tavares, L.R. Monteiro,R.J. Lopes, M.M. Correia dos Santos, R.W. Furness, Bulletin of Environmental Contamination and Toxicology, 72 (2004) 437 doi:10.1007/s00128-004-0265-8

“Lead adsorption on a soil: a polarografic study”
M.A. Trancoso, M.M.Correia dos Santos, M.L.Simões Gonçalves, Electroanalysis, 16 (2004) 1024-1032. doi: 10.1002/elan.200302891

ANTERIOR a 2000

"Determination of stability constants of quasi-reversible systems: copper chlorocomplexes in seawater conditions"
M.L. Simões Gonçalves, M.M. Correia dos Santos, J. Electroanal. Chem., 143 (1983) 397. doi:10.1016/S0022-0728(83)80276-9

"Cadmium complexes of aminoacids in seawater conditions"
M.L. Simões Gonçalves, M.M. Correia dos Santos, J. Electroanal.
Chem., 163 (1984) 315. doi:10.1016/S0022-0728(84)80059-5

"Electroanalytical chemistry of cadmium complexes of aminoacids at the ionic strength of seawater (0.70M NaClO )- Part II"
M.L. Simões Gonçalves, M.M. Correia dos Santos, J. Electroanal. Chem., 187 (1985) 333. doi:10.1016/0368-1874(85)85788-9

"Electroanalytical chemistry of copper, lead and zinc complexes of amino-acids at the ionic strength of seawater (0.70M NaClO4 ). Part III"
M. M. Correia dos Santos, M. L. Simões Gonçalves, J. Electroanal. Chem., 208 (1986) 137. doi:10.1016/0022-0728(86)90302-5 

"Simulations of a Natural Interface"
A. M. Mota, M.L. Simões Gonçalves, M. M. Correia dos Santos, Port.
Elect. Acta, 5 (1987), 191.

"Flow-Injection Titration of Alkalinity in Natural Waters"
D. R. Turner, S. Knox, M. Whitfield, M. M. Correia dos Santos, Analytical Proceedings, 24 (1987) 359-364.  doi:10.1039/AP9872400359

"Adsorption of aminoacids on the mercury electrode and kinetics of dissociation of their Cu(II), Pb(II), Cd(II) and Zn(II) complexes at the ionic strength of seawater (0.70M NaClO4)”
M.L. Simões Gonçalves, M.M. Correia dos Santos, The Science of the Total Env., 76 (1988) 1. doi:10.1016/0048-9697(88)90278-1 

"Application of Cyclic Voltammetry to a CE Mechanism in a Natural Water"
M.L. Simões Gonçalves, M.M. Correia dos Santos, Port. Elect. Acta, 7 (1989) 37.

" An Integrated Gradient Chamber and Potentiometric Detector for flow injection analysis : Part 1. Assessment of the system"
D.R. Turner, S. Knox, M. Whitfield, M.M. Correia dos Santos, C. Pescada, M.L. Gonçalves, Anal.
Chim. Acta, 222 (1989) 229. doi:10.1016/S0003-2670(00)83280-8

" An Integrated Gradient Chamber and Potentiometric Detector for flow injection analysis : Part 2. Measurement of acidity constants of weak acids "
D.R. Turner, S. Knox, M. Whitfield, M.M. Correia dos Santos, C. Pescada, M.L. Gonçalves, Anal.
Chim. Acta, 222 (1989) 239. doi:10.1016/S0003-2670(00)83281-X

"Reduction of Copper(II) in the Presence of Monoamines at the Ionic Strength of Seawater"
M.M. Correia dos Santos, M.L. Simões Gonçalves, Analytical Proceedings, 28 (1991) 72-81 Doi:10.1039/AP9912800072

"Voltammetric Studies of Copper(I) and Copper(II) with Monoamines at the Ionic Strength of the Seawater"
M.M. Correia dos Santos, M.L. Simões Gonçalves, Electroanalysis, 3 (1991) 131. doi:10.1002/elan.1140030210

"Rapid pK Measurements for Multibasic Acids by Gradient FIA Titration",
D.R. Turner, M.M. Correia dos Santos,P.Coutinho, M.L. Simões Gonçalves, S. Knox, Anal. Chim. Acta, 258 (1992) 259. doi:10.1016/0003-2670(92)85100-K 

"Determination of Stability Constants Using a Mercury Microelectrode and Steady-State Voltammetry "
M.M. Correia dos Santos, M.L. Simões Gonçalves, Electrochim. Acta, 37 (1992) 1413. doi:10.1016/0013-4686(92)87015-R

"Determination of Stability Constants Using Normal Pulse Voltammetry at Microelectrodes "
K. Wikial, M.M. Correia dos Santos, J. Osteryoung, Electrochim. Acta, 38 (1993) 1555. doi:10.1016/0013(93)80286-C

"Kinetics of Dissociation of Copper (II) Proline Complexes by Cyclic Voltammetry with NAFION - Coated Electrode"
M.M. Correia dos Santos, Sofia Capelo, M.L.S. Simões Gonçalves, J. Electroanal. Chem., 364 (1994) 171. doi:10.1016/0022-0728(93)02948-H

"Rapid Determination of Complexing Stability Constants by Gradient Flow Injection Titration"
A.L. Conceição, M.L. Simões Gonçalves, M.M. Correia dos Santos, Anal. Chim. Acta, 302 (1995) 97. doi:10.1016/0003-2670(94)00434-N

"Cadmium and Lead Complexation by Anodic Stripping Voltammetry with a Mercury Microelectrode"
M.M. Correia dos Santos, M.L. Simões Gonçalves, S. Capelo, Electroanalysis, 8 (1996) 178 doi:10.1002/elan.1140080215

"Voltammetric Studies of Purine Bases and Purine Nucleosides with Copper(II)"
M.M. Correia dos Santos, C.M.L. Freire Lopes, M.L. Simões Gonçalves, Bioelectrochem. Bioenerg., 39 (1996) 55. doi:10.1016/0302-4598(95)01857-3

"Study of CE mechanisms by square wave voltammetry: Cd(II) nitrilotriacetic acid and Cd(II)- aspartic acid systems"
M. M. Correia dos Santos, M.L. Simões Gonçalves, J.C. Romão, J. Electroanal. Chem., 413 (1996) 97. doi:10.1016/0022-0728(96)04614-1

“Flow injection-assisted optical sensor for determination of iron(II) and iron(III) in natural water”
A.L.Conceição, M.T.Tena, M.M.Correia dos Santos, M.L.Simões Gonçalves, M.D.Luque de Castro, Anal. Chim. Acta, 343 (1997) 191. doi:10.1016/S0003-2670(96)00595-8

“Electrochemical behavior at macro and microelectrodes of cytochromes c and c3 from D.vulgaris - A comparative study”
P.M.Paes de Sousa, M.M.Correia dos Santos, M.L.Simões Gonçalves, Port. Elect. Acta, 15 (1997) 407.

“Copper complexation by cytosine and its nucleoside”
M.M.Correia dos Santos, P.M.P.Sousa, A.M.M.Modesto, M.L.Simões Gonçalves, Bioelectrochem.
Bioenerg, 45 (1998) 267. doi:10.1016/S0302-4598(97)00102-5

"Electrochemical Studies on c-type Cytochromes at Microelectrodes”
M.M. Correia dos Santos, P.M.P. Sousa, M.L. Simões Gonçalves, H. Lopes, I. Moura, J. Moura, J. Electroanal. Chem., 464 (1999) 76. doi:10.1016/S0022-0728(98)00474-4

“Thermodynamics of uptake of Cadmium by Chlorella Marina
M.C.Ezequil Rollemberg, M.L.Simões Gonçalves, M.M.Correia dos Santos, Bioelectrochem. Bioenerg., 48 (1999) 61. doi:10.1016/S0302-4598(98)00220-

“Speciation of copper in the presence of bromazepam”,
V.Famila, M.M.Correia dos Santos, M.L.Simões Gonçalves, Port.
Elect. Acta, 17 (1999) 165.