José Manuel Gaspar Martinho

Full Professor

CQE, IST, Av. Rovisco Pais 1
1049-001 Lisboa, Portugal

phone: (+351) 218 419 250
email: jgmartinho[AT]
Biotin-End-Functionalized Highly Fluorescent Water-Soluble Polymers
Relogio,P.; Bathfield, M.; Haftek-Terreau, Z.; Beija, M.; D’Agosto, F.; Favier, A.; Giraud-Panis, M.-J.; Mandrand, B.; Farinha, J. P. S.*.; Charreyre, M.-T.;* Martinho, J. M. G.,
Polym. Chem., 2013, 4, 2968–2981.

The cover image is a reproduction of the painting "Lucifer Yellow" by Ana Tristany. Four polymer chains (blue) labeled with several LY fluorophores (yellow), each with a biotin at one chain-end (blue/black) interact with a streptavidine protein (red).
Resonance Energy Transfer in Polymer Nanodomains (Review Article)
Martinho, J. M. G.; Farinha, J. P. S.,
Journal of Physical Chemistry C, 2008, 112, 10591-10601.

The cover is a reproduction of the painting "Interfaces" (acrylic on canvas, 1.5 m x 1.5 m) by Ana Tristany. Nano-size domains located at the interface between different polymers (in black and grey) are labeled with energy donor (red) and acceptor (green) dyes.

A Cationic Smart Copolymer for DNA Binding
Ribeiro,T.; Santiago,A.M.; Martinho,J.M.G.; Farinha,J.P.S.*
Polymers 2017, 11, 576

A new copolymer with a temperature-responsive block and a cationic block forms coacervate complexes with the DNA. The new materials offer good prospects for application in controlled gene delivery.
Synthesis and applications of Rhodamine derivatives as fluorescent probes (Review Article)
Beija,M.; Afonso C.A.M; Martinho J. M. G.
Chem. Soc. Rev., 2009, 38, 2410–2433.

This review describes the  strategies for modification of Rhodamine dyes and discuss the applications of these new derivatives as fluorescent probes.
Selected Publications
Dye-labelled polymer chains at specific sites: Synthesis by living/controlled polymerization (Review Article)
Beija, M.; Charreyere, M-T.; Martinho. J. M. G.
Prog. Polym. Sci.,2011, 36, 568-602-

This review highlights several strategies, based on living/controlled polymerization, to introduce at specific chain sites one and only one dye per polymer chain of controlled molecular weight.


Research Interests
Nanohybrid silica/polymer aerogels:The combined influence of polymer nanoparticle size and content.
Fidalgo, A., Farinha, J. P.S., Martinho, J. M. G., Ilharco, L. M.;
Materials and Design, 2020, 189, 108521.

New nanohybrids silica/polymer aerogels monoliths dried at room temperature and ambient pressure were produced. For each nanoparticle size there is a particular content that results in a minimum value of apparent density, maximum porosity and larger mesopore dimensions.
Selective Two-Photon Absorption in Carbon Dots: an extra piece of photoluminescence emission puzzle.
Santos, C.; Mariz, I; Pinto,S.N.; Gonçalves, G.; Bidkin,I. ; Marques, P. A.A.P.;Neves, Neves, M.G. P. M. S.;  Martinho, J.M.G.; Maçôas, E.M.S.
Nanoscale, 2018,10(26),12505. 10.1039/C8NR03365J

Carbon dots were prepared by controlled chemical cutting of graphene oxide (GO) and two-photon absorption in the 700–1000 nm region recorded. At the same excitation energy, nonlinear excitation results in excitation-wavelength independent emission, while upon linear excitation the emission is excitation-wavelength dependent.

Biocompatible hybrids based on nanoGraphene Oxide covalently linked to glycolporphyrins: synthesis, characterization and biological evaluation.
Santos, C.;  Marques, P.;  Gonçalves, G.; Mariz, I.; Martinho, J.M.G.; Maçoas, E.M.S.; Neves, M.G. P. M. S.
Carbon, 2018,135, 202.

New graphene oxide/porpyrin hybrids bearing glycol chains at specific positions of the porphyrin core were synthesized.  The  effects on cell proliferation and viability, the generation of reactive oxygen species as well as the cell morphology after cell uptake were analysed using Saos-2 cells. The  biocompatibility of these hybrids is promising for future application in biomedicine.
A V-shaped cationic dye for nonlinear optical bioimaging.
Maçoas, E.; Marcelo, G.; Pinto, S.; Cañeque, T.; Cuadro, A. M.; Vaquero, J. J.; Martinho, J. M. G.
Chem. Commun., 2011, 47, 7374.

A symmetric cationic molecule with D-pi-A+-pi-D architecture was synthesized with high two-photon absorption cross-section of 1140 GM. Fluorescece microscopy revealed its presence inside the cell staining vesicular shape organelles in the cytoplasm and lifetime imaging microscopy shows that it is also able to penetrate within the nucleus.